Study Guide for Exam 2, 1999

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 since 1 November1999 

Disclaimer: This list is a guide only. There may be other things we covered in class that I forgot to put on the list, and that could be included in the exam!

TYPES OF PROBLEMS YOU SHOULD BE ABLE TO SOLVE

(also use the problem sets as a study guide)

  1. analyze human pedigrees
  2. detect linkage to an RFLP
  3. detect linkage to microsatellite markers
  4. detect disease alleles directly and indirectly
  5. design or analyze crosses involving mutant mice, flies, worms, or yeast
  6. analyze complementation data
  7. analyze linkage data
  8. analyze data in deletion mapping

KNOW HOW and WHEN TO:

  1. clone by complementation
  2. positional clone
  3. Southern blot
  4. Northern blot
  5. analyze expression patterns
  6. do in situ hybridization
  7. analyze DNA by PCR
  8. do somatic and germ-line gene therapy
  9. make a knock-out mouse
  10. find a gene by conserved synteny
  11. screen for dominant mutations
  12. screen for recessive mutations
  13. use balancer chromosomes in maintaining a genetic stock, in comple- mentation testing, and in an F3 screen
  14. use mice, flies, worms, or yeast as model organisms

CONCEPTS TERMS, METHODS, GENETIC TOOLS TO KNOW

  1. molecular basis of dominance and recessiveness
  2. autosomal dominant
  3. autosomal recessive
  4. X-linked dominant
  5. X-linked recessive
  6. penetrance
  7. expressivity
  8. phenocopy
  9. suppressor
  10. plasmid
  11. library
  12. genomic DNA
  13. RNA
  14. restriction enzyme
  15. RFLP
  16. microsatellite marker
  17. PCR
  18. primers/oligonucleotides
  19. LOD score
  20. gel
  21. probe
  22. denature
  23. hybridize
  24. autoradiogram
  25. ES cells
  26. blastula
  27. TK gene
  28. neomycin-resistance (neor) gene
  29. homologous and non-homologous recombination
  30. features of balancer chromosomes
  31. protein motifs
  32. conserved synteny
  33. preimplantation embryos
  34. amneocentesis
  35. model organisms
  36. why the mouse?
  37. why the fly?
  38. why the worm?
  39. why yeast?
  40. larva
  41. pupa
  42. budding yeast
  43. tetrads
  44. antennapedia and bithorax complexes
  45. polytene chromosomes
  46. homeodomain
  47. transcription factor
  48. nonsense and missense mutations