DNA Structure and Population Genetics Practice Problems

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since 26 Feb 2007

Practice Problems, Solutions to be posted

1. Draw the complementary strand for the following DNA
   • 5' A A T T C C G G 3'

2. Consider the following small section of a DNA molecule,
   • 5' A T C G G G G G G G G G G G A A G 3'

   • Suppose you wish to PCR up the G G -- G region. Design the primers (each three bases long) to do so.

3. Suppose the frequencies for alleles 11 and 12 (at Marker 1) are 0.2 and 0.3, and the frequency of allele 6 at Marker 2 is 0.1.
   • Under Hardy-Weinberg and the product rule, what is the probability of an individual being 11,12 and 6,6

   • Now use NRC recommendation 4.1, and assume theta = 0.05. What is this match probability?

   • Now use NRC recommendation 4.2 also with theta = 0.05. What is this match probability (use Eq. 4.10 in the notes)